An isothermal in-vitro nucleotide amplification process. The process involves the concomitant action of a RNA-DIRECTED DNA POLYMERASE, a ribonuclease (RIBONUCLEASES), and DNA-DIRECTED RNA POLYMERASES to synthesize large quantities of sequence-specific RNA and DNA molecules.

Synonyms: Nucleic Acid Sequence Based Amplification, NASBA Analyses, Analyses, NASBA, Self-Sustained Sequence Replication, Sequence Replication, Self-Sustained, Replications, Self-Sustained Sequence, Self Sustained Sequence Replication, Replication, Self-Sustained Sequence, Self-Sustained Sequence Replications, Analysis, NASBA, Sequence Replications, Self-Sustained, NASBA, NASBA Analysis, Nucleic Acid Sequence-Based Amplification